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hoechst 33 342 dna staining solution  (Thermo Fisher)
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Thermo Fisher hoechst 33 342 dna staining solution
Hoechst 33 342 Dna Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pureblutm hoechst 33 342 nuclear staining dye  (Bio-Rad)
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Bio-Rad pureblutm hoechst 33 342 nuclear staining dye
Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with <t>PureBluTM</t> <t>Hoechst</t> 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
Pureblutm Hoechst 33 342 Nuclear Staining Dye, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pureblutm hoechst 33 342 nuclear staining dye/product/Bio-Rad
Average 99 stars, based on 1 article reviews
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dna stain hoechst 33 342  (Thermo Fisher)
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Thermo Fisher dna stain hoechst 33 342
Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with <t>PureBluTM</t> <t>Hoechst</t> 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
Dna Stain Hoechst 33 342, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst 33 342 staining solutions for live cells  (Beyotime)
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Beyotime hoechst 33 342 staining solutions for live cells
Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with <t>PureBluTM</t> <t>Hoechst</t> 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
Hoechst 33 342 Staining Solutions For Live Cells, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

Journal: Cell communication and signaling : CCS

Article Title: Induced mitochondrial deficit by NDUFS3 transient silencing reduces RAB7 expression and causes lysosomal dysfunction in pancreatic cancer cells.

doi: 10.1186/s12964-025-02214-y

Figure Lengend Snippet: Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

Article Snippet: Before seeding, nuclei of cells were stained with 20 nM of PureBluTM Hoechst 33,342 Nuclear Staining Dye (Bio-Rad).

Techniques: Quantitative Proteomics, Western Blot, Transfection, Control, Staining, Labeling, Fluorescence, Software